PrimeQ Real Time PCR Cycler

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PrimeQ real-time PCR system

The PrimeQ real-time PCR system has been designed with an open architecture and chemistry format that allows user flexibility in qPCR methods and research pursuits. The supporting Quansoft® software provides an easy to use comprehensive data analysis package with the flexibility to edit experiment setup even after the run is complete.


PrimeQ Instrument


Key Features


SINGLE WHITE LED LIGHT SOURCE
- Ensures consistent power output to each well compared to tungsten halogen lamps or other multiple LED systems.
- Provides a long term life span unlike tungsten halogen lamps that require frequent replacement.

PMT (PHOTOMULTIPLIER TUBE) DETECTOR
- PMT is designed for accurate detection of photons compared to CCD cameras which are just an imaging system.
- PMT provides raw data for analysis that can be manipulated directly whereas CCD uses image comparisons interpreted by internal algorithms.

FLEXIBLE SCANNING MECHANISM
- Allows both partial and full plate reads.
- High accuracy ensures no cross talk between well reads.
- Full plate read in 20 seconds per filter.

MULTIPLEX CAPABILITIES
- 4 paired excitation and emission filters housed in an individual cartridge system.
- Open system to preferred chemistry and not locked in to factory calibrated dyes.

HEATED LID
- The flexible optical heated lid system is designed to minimize loss of sample and prevent sample condensation.
- Temperature controlled, it allows further method optimization where pre-incubation stages may be required (e.g. in reverse transcription techniques) or where changes in the heated lid characteristics are needed during the different stages of a program run.


Quansoft® software and reporting


Accompanying PrimeQ is our easy-to-use, flexible software Quansoft®, which gives you the freedom to manipulate your raw data, choose your own chemistries and consumables.


Key Features


- Ability to create, modify and save files using Quansoft® while a program is running on the unit
- Ability to run without initially defining your plate layout and analysis criteria - just pop a prepared plate in & run a program
- Ability to export unprocessed, raw data
- Install Quansoft® on multiple PCs without paying a license fee (only a simple registration is required)
- Ability to change from first order derivative to fit points in results analysis


Technical Specifications


Parameter PrimeQ
Block format 96 x 0.2ml
Block specification 8 x peltier elements per block. Quad-circuit technology to enhance performance
Block uniformity at 50°C ± 0.25°C
Maximum ramp rate Up to 2.2°C
Temperature range 4 to 98°C
Sample volume 15 to 50ul
Heated lid Adjustable between 100 and 115°C in 1°C increments, or off
Maximum sample evaporation Less than 15 volume loss from any well using the plate sealing recommended by Techne
PC connectivity USB, OS Win XP SP3 or higher and Win 7 (one unit per PC)
Plate format Low profile 96-well plate (skirted or non-skirted)
Excitation source Solid state white light source
Detector Photon counting photomultiplier tube
Multiplex dye detection Up to 4 dyes per reaction tube
User chosen filters Maximum of 4 paired excitation emission filter cartridge systems suitable wth currently used dyes*
Fluorescence excitation range 470-650nm (standard filters are centered at 485, 530, 580 and 640nm)
Fluorescence detection range 500-710nm (standard filters are centered at 520, 560, 615 and 685nm)
Dynamic range At least 9 orders of magnitude of target DNA concentration
Sensitivity (detection threshold) 1nM fluorescein in a 20ul sample
Resolution 1.5 fold difference in target DNA concentration
Weight 25kg
Dimensions (WxHxD) 407 x 508 x 534mm
Input power 715W
Power supply 100-230V, 50-60Hz


*custom filter cartridge systems available upon request


Ordering Information


Product Code Description
PrimeQ PrimeQ real-time PCR system
FC01* FAM multiplex, 460nm excitation wavelength, 500nm emission wavelength
FC02 Green, FAM, 485nm excitation wavelength, 520nm emission wavelength
FC03 Yellow, HEX, 530nm excitation wavelength, 560nm emission wavelength
FC04 Red, ROX, 580nm excitation wavelength, 615nm emission wavelength
FC05 Blue, Cy5, 640nm excitation wavelength, 685nm emission wavelength


*Please note FC01 is not included with the unit. FC02-FC05 are included with the unit

Bibby reserve the right to make specification changes at any time.
However, the information on this website is correct at time of reading.


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