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PrimeQ real-time PCR system

The PrimeQ real-time PCR system has been designed with an open architecture and chemistry format that allows user flexibility in qPCR methods and research pursuits. The supporting Quansoft® software provides an easy to use comprehensive data analysis package with the flexibility to edit experiment setup even after the run is complete.

Key Features

SINGLE WHITE LED LIGHT SOURCE
- Ensures consistent power output to each well compared to tungsten halogen lamps or other multiple LED systems.
- Provides a long term life span unlike tungsten halogen lamps that require frequent replacement.

PMT (PHOTOMULTIPLIER TUBE) DETECTOR
- PMT is designed for accurate detection of photons compared to CCD cameras which are just an imaging system.
- PMT provides raw data for analysis that can be manipulated directly whereas CCD uses image comparisons interpreted by internal algorithms.

FLEXIBLE SCANNING MECHANISM
- Allows both partial and full plate reads.
- High accuracy ensures no cross talk between well reads.
- Full plate read in 20 seconds per filter.

MULTIPLEX CAPABILITIES
- 4 paired excitation and emission filters housed in an individual cartridge system.
- Open system to preferred chemistry and not locked in to factory calibrated dyes.

HEATED LID
- The flexible optical heated lid system is designed to minimize loss of sample and prevent sample condensation.
- Temperature controlled, it allows further method optimization where pre-incubation stages may be required (e.g. in reverse transcription techniques) or where changes in the heated lid characteristics are needed during the different stages of a program run.

Quansoft® software and reporting

Accompanying PrimeQ is our easy-to-use, flexible software Quansoft®, which gives you the freedom to manipulate your raw data, choose your own chemistries and consumables.

Key Features

- Ability to create, modify and save files using Quansoft® while a program is running on the unit
- Ability to run without initially defining your plate layout and analysis criteria - just pop a prepared plate in & run a program
- Ability to export unprocessed, raw data
- Install Quansoft® on multiple PCs without paying a license fee (only a simple registration is required)
- Ability to change from first order derivative to fit points in results analysis

Technical Specifications

Parameter	PrimeQ
Block format	96 x 0.2ml
Block specification	8 x peltier elements per block. Quad-circuit technology to enhance performance
Block uniformity at 50°C	± 0.25°C
Maximum ramp rate	Up to 2.2°C
Temperature range	4 to 98°C
Sample volume	15 to 50ul
Heated lid	Adjustable between 100 and 115°C in 1°C increments, or off
Maximum sample evaporation	Less than 15 volume loss from any well using the plate sealing recommended by Techne
PC connectivity	USB, OS Win XP SP3 or higher and Win 7 (one unit per PC)
Plate format	Low profile 96-well plate (skirted or non-skirted)
Excitation source	Solid state white light source
Detector	Photon counting photomultiplier tube
Multiplex dye detection	Up to 4 dyes per reaction tube
User chosen filters	Maximum of 4 paired excitation emission filter cartridge systems suitable wth currently used dyes*
Fluorescence excitation range	470-650nm (standard filters are centered at 485, 530, 580 and 640nm)
Fluorescence detection range	500-710nm (standard filters are centered at 520, 560, 615 and 685nm)
Dynamic range	At least 9 orders of magnitude of target DNA concentration
Sensitivity (detection threshold)	1nM fluorescein in a 20ul sample
Resolution	1.5 fold difference in target DNA concentration
Weight	25kg
Dimensions (WxHxD)	407 x 508 x 534mm
Input power	715W
Power supply	100-230V, 50-60Hz

*custom filter cartridge systems available upon request

Ordering Information

Product Code	Description
PrimeQ	PrimeQ real-time PCR system
FC01*	FAM multiplex, 460nm excitation wavelength, 500nm emission wavelength
FC02	Green, FAM, 485nm excitation wavelength, 520nm emission wavelength
FC03	Yellow, HEX, 530nm excitation wavelength, 560nm emission wavelength
FC04	Red, ROX, 580nm excitation wavelength, 615nm emission wavelength
FC05	Blue, Cy5, 640nm excitation wavelength, 685nm emission wavelength