PrimeQ Real Time PCR Cycler

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PrimeQ real-time PCR system

The PrimeQ real-time PCR system has been designed with an open architecture and chemistry format that allows user flexibility in qPCR methods and research applications. The supporting Quansoft® software provides an easy to use comprehensive data analysis package with the flexibility to edit experiment setup even after the run is complete.

Key Features

Ensures consistent excitation to each well compared to tungsten halogen lamps. LED has a long lifespan unlike halogen lamps which require frequent replacement.

PMT are designed for a more accurate detection of photons compared to CCD imaging systems. PMT provides raw fluorescence data for analysis which can be manipulated directly without the need for interpretation by internal algorithms.

Allows partial plate reads to save time. High accuracy ensures no cross talk between wells. A full 96 well plate is read in 20 seconds per filter.

4 paired excitation and emission filters are housed in individual cartridges. PrimeQ is open to use any preferred chemistry and is not locked in to factory calibrated dyes.

The optical heated lid system is designed to minimize loss of sample and prevent condensation. Temperature controlled, it allows method optimization where pre-incubation stages are required (e.g. reverse transcription techniques) or where changes in the heated lid characteristics are needed during different stages of a program run.

Quansoft® software and reporting

Accompanying PrimeQ is our easy-to-use, flexible software Quansoft®, which gives you the freedom to manipulate your raw data, choose your own chemistries and consumables.

Key Features

- Ability to create, modify and save files using Quansoft® while a program is running on the unit
- Ability to run without initially defining your plate layout and analysis criteria - just insert a prepared plate and run a program
- Ability to export unprocessed, raw data
- Install Quansoft® on multiple PCs without paying a license fee
- Ability to change from first order derivative to fit points in results analysis

Technical Specifications

Parameter PrimeQ
Block format 96 x 0.2ml
Block specification Aluminium. 8 x peltier elements per block
Block uniformity at 50°C ± 0.25°C
Maximum ramp rate 2.2°C per second
Temperature range 4 to 98°C
Sample volume 10 to 50ul
Heated lid Adjustable between 100 and 115°C in 1°C increments, or off
PC connectivity USB, OS Win XP SP3 or higher and Win 7 (one unit per PC)
Plate format Low profile 96-well plate (skirted or non-skirted)
Excitation source Solid state white light source
Detector Photon counting photomultiplier tube
Multiplex dye detection Up to 4 dyes per reaction tube
Filter cartridges 4 paired excitation and emission filter cartridges are provided
Fluorescence excitation range 470-650nm (standard filters are centered at 485, 530, 580 and 640nm)
Fluorescence detection range 500-710nm (standard filters are centered at 520, 560, 615 and 685nm)
Dynamic range At least 9 orders of magnitude of target DNA concentration
Sensitivity (detection threshold) 1nM fluorescein in a 20ul sample
Resolution 1.5 fold difference in target DNA concentration
Dimensions (WxHxD) 407 x 508 x 534mm
Weight 25kg
Input power 715W
Power supply 100-230V, 50-60Hz

Ordering Information

Product Code Description
PrimeQ PrimeQ real-time PCR system
FC01* FAM multiplex, 460nm excitation wavelength, 500nm emission wavelength
FC02 Green, FAM, 485nm excitation wavelength, 520nm emission wavelength
FC03 Yellow, HEX, 530nm excitation wavelength, 560nm emission wavelength
FC04 Red, ROX, 580nm excitation wavelength, 615nm emission wavelength
FC05 Blue, Cy5, 640nm excitation wavelength, 685nm emission wavelength

*Please note FC01 is not included with the unit. FC02-FC05 are included with the unit

Bibby reserve the right to make specification changes at any time.
However, the information on this website is correct at time of reading.

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